505
Feeding guinea pigs with emulsions of plague cadavers, and cultures of the B. pestis, mixed with boiled ground rice, and dried over sulphuric acid. In the case of tissues, the organs or pieces selected for experiment were previously ground up in a mortar, and then well mixed with the rice in sterilised plate dishes. The cultures - both bouillon and agar-agar - were in every case 48 hours old and incubated at 36-37°C. They were poured over the rice and thoroughly mixed, with clean glass rods, through the rice, before placing the dishes before the animals. In order to put to the test the exact value of Klein's results with dried tissues and cultures, and small doses or amounts uniformly, culture and tissue were used uniformly throughout the experiments.
The details, as regards technique, are mainly similar to that already described under Series, No. I.
Table No. II. is a protocol of the main experiments.
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17.
505
Feeding guinea pigs with ngaus and Timues of plague cadavers, and Cultures of the B. pestis, mixed with boiled Ground rice, and dried over Sulphuric Acid. In the case
and tissues, the
1
the
organs
pieces selected for Experiment were
previously ground up in a mortar, and then will mixed with the rice in steriliser plate dishes The Cultures - both bouillow and agar-agar
- were inn
wery case 48 hours, old and incubated at 36-87°C. They were pouted over the rice and thoroughly mixed, with dean glass rods, through the vice, before flacing the dishes before the animals. In order to put to the list, the weact value of Kleins results with dried hisws and cultures, and small doses or amounts
niformly
culture and tissue were used un
throughout the Experiments.
he one details, as regards technique,
are madtly punilar It that ahead described under Series, No.I.
Pable. No. II. is a
Protocol of the mains
..Experiments
Page 510Page 511
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